Optimising Exome Captures in Species With Large Genomes Using Species-Specific Repetitive DNA Blocker.

ABSTRACT: Large and highly repetitive genomes are common. However, research interests usually lie within the non-repetitive parts of the genome, as they are more likely functional, and can be used to answer questions related to adaptation, selection and evolutionary history. Exome capture is a cost-effective method for providing sequencing data from protein-coding parts of the genes. C0t-1 DNA blockers consist of repetitive DNA and are used in exome captures to prevent the hybridisation of repetitive DNA sequences to capture baits or bait-bound genomic DNA. Universal blockers target repetitive regions shared by many species, while species-specific c0t-1 DNA is prepared from the DNA of the studied species, thus perfectly matching the repetitive DNA contents of the species. So far, the use of species-

SUBMITTER: Kesalahti R 

PROVIDER: S-EPMC11887611 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature