Ocular Surface Involvements in the Development of Sjogren's Syndrome-Associated Dry Eye in the IL14α Transgenic Mouse.

ABSTRACT:

Purpose

To investigate the ocular surface changes during progress of the Sjögren's Syndrome (SS), using a previously described IL14α transgenic mice (IL14α TG) SS model.

Methods

The ocular surface of IL14α TG and C57BL/6 wild-type (WT) female mice were evaluated at the age of six, nine, 12, 15, and 18 months. Slit lamp microscopy observation, Oregon green dextran staining, Schirmer test, and periodic-acid-Schiff staining were assessed. Immunohistochemistry, immunofluorescence, and associated gene expression analysis by qPCR and ELISA were performed in cornea, conjunctiva, and lacrimal grand at different ages of the mice. Masson's trichome staining was conducted on lacrimal gland cryosections.

Results

Compared with C57BL/6 WT mice, IL14α TG mice showed corneal barrier

SUBMITTER: Zhang M 

PROVIDER: S-EPMC11887930 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature