Influence of Hypoxia on a Biomaterial Model of the Bone Marrow Perivascular Niche.

ABSTRACT: Hematopoietic stem cell (HSC) fate is shaped by distinct microenvironments termed niches within the bone marrow. Quiescence, expansion, and differentiation are directly and indirectly regulated by complex combinations of cell secretomes, cell-cell interactions, mechanical signals, and metabolic factors including oxygen tension. The perivascular environment in the bone marrow has been implicated in guiding HSC fate. However, bone marrow presents an environment which is hypoxic (~1-4% O₂) relative to traditional cell culture conditions, and the study of hypoxia in vitro is complicated by the speed with which normoxic conditions during HSC isolation induce differentiation. There is a unique opportunity to use engineered models of the bone marrow to investigate the impact of defined

SUBMITTER: Thompson GB 

PROVIDER: S-EPMC11888168 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature