Fiber photometry analysis of spontaneous dopamine signals: The z-scored data are not the data.

ABSTRACT: Fluorescent sensors have revolutionized the measurement of molecules in the brain, and the dLight dopamine sensor has been used extensively to examine reward- and cue-evoked dopamine release, but only recently has the field turned its attention to spontaneous release events. Analysis of spontaneous events typically requires evaluation of hundreds of events over minutes to hours, and the most common method of analysis, z-scoring, was not designed for this purpose. Here, we compare the accuracy and reliability of three different analysis methods to identify pharmacologically induced changes in dopamine release and uptake in freely moving C57BL/6J mice. The D1-like receptor antagonist SCH23390 was used to prevent dLight sensors from interacting with dopamine in the extracellular space, while

SUBMITTER: Wallace CW 

PROVIDER: S-EPMC11888193 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature