Real-Time and Site-Specific Perturbation of Dynamic Subcellular Compartments Using Femtosecond Pulses.

ABSTRACT: Understanding laser interactions with subcellular compartments is crucial for advancing optical microscopy, phototherapy, and optogenetics. While continuous-wave (CW) lasers rely on linear absorption, femtosecond (fs) lasers enable nonlinear multiphoton absorption confined to the laser focus, offering high axial precision. However, current fs laser delivery methods lack the ability to target dynamic molecular entities and automate target selection, limiting real-time perturbation of biomolecules with mobility or complex distribution. Additionally, existing technologies separate fs pulse delivery and imaging, preventing simultaneous recording of cellular responses. To overcome these challenges, we introduce fs real-time precision opto-control (fs-RPOC), which integrates a laser scanning mic

SUBMITTER: Ma S 

PROVIDER: S-EPMC11888216 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature