3D spatial transcriptomics reveals the molecular structure of input and output pathways in the mouse olfactory bulb.

ABSTRACT: A core organizing principle of the vertebrate brain is its symmetry along multiple axes. However, the precision with which neurons, circuit modules, and brain regions align to these axes remains poorly understood. Here, we used 3D spatial transcriptomics to reconstruct the anatomical and molecular organization of the mouse olfactory bulb. We mapped the positions of nearly one thousand molecularly distinct glomeruli, the structural and functional units of odor processing, revealing highly symmetric organization across hemispheres. Within each bulb, we delineated a curved axis of symmetry that divides pairs of sister glomeruli. Gene expression in the olfactory epithelium predicted glomerular position with near-glomerular resolution. However, glomerular symmetry did not extend to deeper layer

SUBMITTER: Klimpert N 

PROVIDER: S-EPMC11888228 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature