Deconvolution to restore cryo-EM maps with anisotropic resolution.

ABSTRACT: With technological advancements in recent years, single particle cryogenic electron microscopy (cryo-EM) has become a major methodology for structural biology. Structure determination by single particle cryo-EM is premised on randomly orientated particles embedded in thin layer of vitreous ice to resolve high-resolution structural information in all directions. Otherwise, preferentially distributed particle orientations will lead to anisotropic resolution of the structure. Here we established a deconvolution approach, named AR-Decon, to computationally improve the quality of three-dimensional maps with anisotropic resolutions reconstructed from datasets with preferred orientations. We have tested and validated the procedure with both synthetic and experimental datasets and compared its per

SUBMITTER: Li J 

PROVIDER: S-EPMC11888254 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature