Regulation of Steady State Ribosomal Transcription in <i>Mycobacterium tuberculosis</i>: Intersection of Sigma Subunits, Superhelicity, and Transcription Factors.

ABSTRACT: The regulation of ribosomal RNA (rRNA) is closely tied to nutrient availability, growth phase, and global gene expression, serving as a key factor in bacterial adaptability and pathogenicity. Mycobacterium tuberculosis (Mtb) stands out from other species with a single ribosomal operon controlled by two promoters: rrnAP3 and rrnAP1 and a high ratio of sigma (σ) factors to genome size. While the primary σ factor σ^A is known to drive ribosomal transcription, the alternative σ factor σ^B has been proposed to contribute to the transcription of housekeeping genes, including rRNA under a range of conditions. However, σ^B's precise role remains unclear. Here, we quantify steady-state rates in reconstituted transcription reactions and establish th

SUBMITTER: Ruiz Manzano A 

PROVIDER: S-EPMC11888270 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature