In-cell Structure and Variability of Pyrenoid Rubisco.

ABSTRACT: Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a key enzyme in the global carbon cycle, catalyzing CO₂ fixation during photosynthesis. To overcome Rubisco's inherent catalytic inefficiency, many photosynthetic organisms have evolved CO₂-concentrating mechanisms. Central to these mechanisms is the pyrenoid, a protein-dense organelle within the chloroplast of eukaryotic algae, which increases the local concentration of CO₂ around Rubisco and thereby enhances its catalytic efficiency. Although the structure of Rubisco has been extensively studied by in vitro methods such as X-ray crystallography and single particle cryo-EM, its native structure within the pyrenoid, its dynamics, and its association with binding partners remain elusive. Her

SUBMITTER: Elad N 

PROVIDER: S-EPMC11888406 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature