Distinct roles for thymic stromal lymphopoietin (TSLP) and IL-33 in experimental eosinophilic esophagitis.

ABSTRACT:

Rationale

Thymic stromal lymphopoietin (TSLP) and IL-33 are alarmins implicated in EoE pathogenesis by activating multiple cells including mast cells (MCs). Whether TSLP or IL-33 have a role in EoE and whether their activities are distinct requires further investigation.

Methods

Experimental EoE was induced in wild type (WT) Il33 ^-/- and Crlf2 ^-/- mice. TSLP or IL-5 were neutralized using antibodies. Esophageal histopathology was determined by H&E, anti-Ki67, anti-CD31 and anti-MBP staining. Esophageal RNA was subjected to RNA sequencing. Bone marrow-derived MCs were activated with TSLP and IL-13 was determined (ELISA).

Results

TSLP and IL-33 were overexpressed in human and experimental EoE. Human and mouse esophageal MCs displayed

SUBMITTER: Dsilva A 

PROVIDER: S-EPMC11888463 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature