Single-nucleus transcriptomics reveal the cytological mechanism of conjugated linoleic acids in regulating intramuscular fat deposition.

ABSTRACT: Conjugated linoleic acids (CLAs) can serve as a nutritional intervention to regulate quality, function, and fat infiltration in skeletal muscles, but the specific cytological mechanisms remain unknown. Here, we applied single-nucleus RNA-sequencing (snRNA-seq) to characterize the cytological mechanism of CLAs regulates fat infiltration in skeletal muscles based on pig models. We investigated the regulatory effects of CLAs on cell populations and molecular characteristics in pig muscles and found CLAs could promote the transformation of fast glycolytic myofibers into slow oxidative myofibers. We also observed three subpopulations including SCD⁺/DGAT2⁺, FABP5⁺/SIAH1⁺, and PDE4D⁺/PDE7B⁺ subclusters in adipocytes and CLAs could

SUBMITTER: Wang L 

PROVIDER: S-EPMC11888599 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature