Extravascular coagulation regulates haemostasis independently of activated platelet surfaces in an in vivo mouse model.

ABSTRACT: While the conventional understanding of haemostatic plug formation is that coagulation proceeds efficiently on the surface of activated platelets at the vascular injury site to form a robust haemostatic plug, this understanding does not explain the clinical reality that platelet dysfunction results in a mild bleeding phenotype, whereas coagulation disorders exhibit severe bleeding phenotypes, particularly in deep tissues. Here, we introduce an in vivo imaging method to observe internal bleeding and subsequent haemostatic plug formation in mice and report that haemostatic plug formation after internal bleeding, coagulation occurs primarily outside the blood vessel rather than on platelets. Experiments in mice with impaired platelet surface coagulation, depleted platelets, haemophilia A or r

SUBMITTER: Sakata A 

PROVIDER: S-EPMC11889134 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature