A new approach for the detection of genetic alterations utilizing modified loop-mediated isothermal amplification reaction (LAMP).

ABSTRACT: The increasing use of genetic testing for personalised therapy, highlights the need for rapid, reliable diagnostics. Current methods are hindered by complex workflows, requiring advanced equipment, skilled personnel, and invasive tissue sampling. Loop-mediated isothermal amplification (LAMP) has emerged as a more efficient alternative to traditional PCR. LAMP eliminates thermal cycling, allowing faster, more cost-effective tests, and is less sensitive to inhibitors, enabling testing from minimally processed samples. Although LAMP is newer and has a longer assay development time than PCR, its potential in oncology, particularly for detecting genetic changes, is promising. We have developed a LAMP-based method for detecting genetic variations, optimized for point-of-care testing. This techni

SUBMITTER: Cierzniak A 

PROVIDER: S-EPMC11889157 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature