Quantitative Chromatin Protein Dynamics During Replication Origin Firing in Human Cells.

ABSTRACT: Accurate genome duplication requires a tightly regulated DNA replication program that relies on the fine regulation of origin firing. While the molecular steps involved in origin firing have been determined predominantly in budding yeast, the complexity of this process in human cells has yet to be fully elucidated. Here, we describe a straightforward proteomics approach to systematically analyze protein recruitment to the chromatin during induced origin firing in human cells. Using a specific inhibitor against CHK1 kinase, we induced a synchronized wave of dormant origin firing (DOF) and assessed the S phase chromatin proteome at different time points. We provide time-resolved loading dynamics of 3269 proteins, including the core replication machinery and origin firing factors. This datase

SUBMITTER: Gadi SA 

PROVIDER: S-EPMC11889381 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature