Establishing a cryopreserved biobank of living tumor tissues for drug sensitivity testing.

ABSTRACT: The cryopreservation of cancer tissues to generate frozen libraries is a common practice used worldwide for storing patient samples for later applications. However, frozen samples stored by existing methods cannot be used for initiating living cell cultures, such as patient-derived tumor organoids (PDOs), which offer great potential for personalized treatment. To overcome this challenge, we developed a novel procedure for culturing PDOs using frozen live tumor tissues. We show that tumor specimens stored using this technique maintain their viability and can be successfully used to generate organoids even after long-term freezing, with an impressive success rate of 95.2 %. Importantly, we found that the structural features, tumor marker expression, and drug responses of organoids derived fr

SUBMITTER: Chen P 

PROVIDER: S-EPMC11889390 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature