Development of an orthotopic medulloblastoma zebrafish model for rapid drug testing.

ABSTRACT:

Background

Medulloblastoma (MB) is one of the most common malignant brain tumors in children. Current preclinical in vivo model systems for MB have increased our understanding of molecular mechanisms regulating MB development. However, they may not be suitable for large-scale studies. The aim of this study was to investigate if a zebrafish-based xenograft model can recapitulate MB growth and enable rapid drug testing.

Methods

Nine different MB cell lines or patient-derived cells were transplanted into blastula-stage zebrafish embryos. Tumor development and migration were then monitored using live imaging. RNA sequencing was performed to investigate transcriptome changes after conditioning cells in a neural stem cell-like medium. Furthermore, drug treatments were tested in a

SUBMITTER: van Bree N 

PROVIDER: S-EPMC11889719 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature