The role of myeloid cell heterogeneity during spontaneous choroidal neovascularization in Vldlr knockout mice.

ABSTRACT:

Background

Myeloid cells are heterogeneous cells that are critical for spontaneous choroidal neovascularization (CNV) in the Vldlr^-/- mouse model. However, the specific myeloid cell subtype necessary for CNV remains unknown.

Methods and results

To investigate the role of monocytes, we bred Ccr2^-/- and Nr4a1^-/- mice into the Vldlr^-/- background. We found that Ccr2 and Nr4a1 deficiency had no effect upon macrophage counts, CNV lesion number, or total CNV area. Next, we investigated the role of microglia by generating Vldlr^-/-Tmem119^CreER/+Rosa26^DTR/+ mice. Diphtheria toxin (DT) treatment reduced macrophage counts at CNV lesions and CNV lesion number, but did not affect total CNV lesion area. To target

SUBMITTER: Rajesh A 

PROVIDER: S-EPMC11889776 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature