Specific sDMA modifications on the RGG/RG motif of METTL14 regulate its function in AML.

ABSTRACT:

Background

Protein arginine methylations are crucial post-translational modifications (PTMs) in eukaryotes, playing a significant regulatory role in diverse biological processes. Here, we present our investigation into the detailed arginine methylation pattern of the C-terminal RG-rich region of METTL14, a key component of the m6A RNA methylation machinery, and its functional implications in biology and disease.

Methods

Using ETD-based mass spectrometry and in vitro enzyme reactions, we uncover a specific arginine methylation pattern on METTL14. RNA methyltransferase activity assays were used to assess the impact of sDMA on METTL3:METTL14 complex activity. RNA immunoprecipitation was used to evaluate mRNA-m6A reader interactions. MeRIP-seq analysis was used to study the geno

SUBMITTER: Zhong Y 

PROVIDER: S-EPMC11889898 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature