A multi-channel CRISPR-based method for rapid, sensitive detection of four diseases of <i>Brassica rapa</i> in the field.

ABSTRACT: Pathogens significantly restrict the production of Brassica rapa (B. rapa L. ssp. Pekinensis), with climate change and evolving planting patterns exacerbating disease prevalence. Multichannel rapid diagnostic methods in the field can facilitate the early detection and control of diseases in B. rapa. Here, we established a multichannel lateral flow biosensor (LFB) combined with a CRISPR/Cas12a cleavage assay for the simultaneous detection of four B. rapa diseases. Key innovations of this study include: (1) High specificity and sensitivity, down to pathogen concentrations of 1.5 pg/μl-due to the optimization of crRNA secondary structure: the more stable the crRNA, the higher its detection sensitivity. (2) Optimized visual detection parameters. We identified ideal

SUBMITTER: Liu X 

PROVIDER: S-EPMC11890027 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature