Improved methodology to survey veterinary antibiotics in environmental samples using µSPEed microextraction followed by ultraperformance liquid chromatography.

ABSTRACT: A μSPEed microextraction combined with ultra-performance liquid chromatography (UHPLC) with UV detection was developed for analysing six veterinary antibiotics (tetracycline, chlortetracycline, oxytetracycline, doxycycline, sulfamethoxazole, and trimethoprim) in environmental samples. To optimise extraction, 12 sorbent cartridges, sample loading cycles, volumes, and pH were assayed. The PS/DVB-RP cartridge, three 250 μL sample loading cycles, and two 50-µL elutions with acidified methanol yielded maximum efficiency. The method was validated with optimised fast chromatographic separation, showing good linearity (R2 > 0.99), precision (RSD < 20%), and recoveries between 46-86%. Detection and quantification limits ranged from 0.30-1.23 μg L^-1 and 0.92-3.73 μg L^-1, respec

SUBMITTER: Antos J 

PROVIDER: S-EPMC11890768 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature