Permeabilisation of the Outer Membrane of Escherichia coli for Enhanced Transport of Complex Molecules.

ABSTRACT: The bacterial envelope plays a critical role in maintaining essential cellular functions by selectively regulating import and export. The selectivity of this envelope can restrict the utilisation of externally provided compounds, thereby restricting the functional space of cellular engineering. This study systematically investigates the potential of large pore outer membrane proteins (OMPs) to enhance outer membrane permeability for diverse challenging compounds. We focus on the general porin OmpF, which facilitates the diffusion of water and small molecules, and specific OMP transporters FhuA and FepA, which mediate the translocation of small hydrophilic compounds. Through comprehensive characterisation, we evaluate the effects of recombinant expression of OMPs and engineered variants for

SUBMITTER: Casas-Rodrigo I 

PROVIDER: S-EPMC11891017 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature