Eggs of Schistosoma japonicum deposited in the spleen induce apoptosis of splenic T cells in C57BL/6 mice.

ABSTRACT: To explore the relationship between Schistosoma japonicum egg deposition and the resultant structural damage to the spleen, mice were infected percutaneously with cercariae or eggs were surgically injected into their spleens. Terminal transferase dUTP nick-end-labeling (TUNEL) showed that cells around the S. japonicum eggs were apoptotic in vivo. Flow cytometry revealed a sharp reduction in splenic B and T cells at 8 weeks post-infection (p.i.) and a significant increase in Annexin V positive T cells. Immunochemistry showed that the remaining follicles in the spleen at 16 weeks p.i. comprised mainly B lymphocytes. Comparing T lymphocytes in the spleen and liver egg granulomas showed obvious CD3⁺ positive areas in the spleen, indicating that splenic egg granulomas have a differen

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11891099 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature