<i>Cellulophaga algicola</i> alginate lyase and <i>Pseudomonas aeruginosa</i> Psl glycoside hydrolase inhibit biofilm formation by <i>Pseudomonas aeruginosa</i> CF2843 on three-dimensional aggregates of lung epithelial cells.

ABSTRACT: Pseudomonas aeruginosa is an opportunistic pathogen that produces a biofilm containing the polysaccharides, alginate, Psl, and Pel, and causes chronic lung infection in cystic fibrosis patients. Others and we have previously explored the use of alginate lyases in inhibiting P. aeruginosa biofilm formation on plastic and lung epithelial cell monolayers. We now employ a more physiologically representative model system, i.e., three-dimensional aggregates of A549 lung epithelial cells cultured under conditions of microgravity in a rotary cell culture system to mimic the natural lung environment, and a previously isolated clinical strain, Pseudomonas aeruginosa CF2843 that we engineered by transposon-mediated integration to express Green Fluorescent Protein and for which we

SUBMITTER: Neetu 

PROVIDER: S-EPMC11891150 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature