Activation of SIRT1 by Hydroxysafflor Yellow A Attenuates Chronic Unpredictable Mild Stress-Induced Microglia Activation and Iron Death in Depressed Rats.

ABSTRACT:

Background

Hydroxysafflor yellow A (HSYA), the main active ingredient in safflower, possesses antioxidant and anti-inflammatory activities. We confirmed in our previous study that HSYA exerts antidepressant effects, but further investigation is needed to uncover the exact mechanism. Herein, we aimed to explore the antidepressant effects of HSYA based on microglial activation and ferroptosis studies.

Methods

The chronic unpredictable mild stress (CUMS) procedure was used to establish a depression model in rats. Behavioral tests were conducted on rats after HSYA administration. Iba-1 immunostaining was used to determine the activation of microglia in the hippocampus. We examined the iron ion level using a colorimetric method. Assayed by western blot for protein expression.

SUBMITTER: He J 

PROVIDER: S-EPMC11891294 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature