Identification of diagnostic and prognostic genetic alterations in uveal melanoma using RNA sequencing.

ABSTRACT: Uveal melanoma is a lethal intraocular tumour, in which the presence of various genetic alterations correlates with the risk of metastatic dissemination and survival. Here, we tested the detectability of all key mutations and chromosomal changes from RNA sequencing data in 80 primary uveal melanomas studied by The Cancer Genome Atlas (TCGA) initiative, and in five prospective cases. Whereas unsupervised gene expression profiling strongly indicated the presence of chromosome 3 alterations, it was not reliable in identifying other alterations. Though, the presence of both chromosome 3 and 8q copy number alterations could be successfully inferred from expressed allelic imbalances of heterozygous common single nucleotide polymorphisms. Most mutations were adequately recognised in the RNA by th

SUBMITTER: Nell RJ 

PROVIDER: S-EPMC11891316 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature