Polo-like kinase2 regulates renal tubulointerstitial fibrosis via notch signaling pathway in diabetic kidney disease.

ABSTRACT: Renal tubulointerstitial fibrosis is considered as an important pathological feature of diabetic kidney disease (DKD). However, the underlying mechanism remains unclear. Polo-like kinase2 (PLK2) is a known player in the regulation of organ fibrosis. Herein, we investigated the expression and function of PLK2 in renal tubular epithelial cells in DKD. Data from the GSE30529 datasets were subjected to analyze the differentially expressed genes (DEGs) in non-diabetic and diabetic renal tubule samples. Molecular docking analysis and Co-IP assay were performed to investigate the interaction between PLK2 and NOTCH1. Immunohistochemistry, immunofluorescent staining, qRT-PCR, and western blot were performed. Our research revealed an increased expression of PLK2 in both DKD mouse kidney tissues and

SUBMITTER: Luo J 

PROVIDER: S-EPMC11891471 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature