Selection of Plastic-Binding DNA Aptamers for Microplastics Detection.

ABSTRACT: Plastics are critical materials for modern technological applications, yet environmental contamination by microplastics has become a growing concern. In this study, DNA aptamers were isolated for two of the most abundant plastic materials: polyvinylchloride (PVC) and polystyrene (PS). These aptamers contain approximately 90 % cytosine and thymine but only 10 % purine content. Among them, the PVC-1 aptamer binds to PVC with a six-fold higher capacity than a random sequenced DNA. Among the tested plastic materials, PVC and PS exhibited the highest specific binding capacity. Using fluorophore-labeled PVC-1 aptamer, PS/PVC microplastics as low as 1 mg were detected, and the aptamer was selective for microplastics over other environmentally relevant materials, such as silica. Molecular dynamics

SUBMITTER: Zandieh M 

PROVIDER: S-EPMC11891633 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature