Proteome-wide identification of druggable targets and inhibitors for multidrug-resistant <i>Pseudomonas aeruginosa</i> using an integrative subtractive proteomics and virtual screening approach.

ABSTRACT: Pseudomonas aeruginosa, a versatile and antibiotic-resistant gram-negative pathogen, poses a critical threat to both immunocompromised and immunocompetent populations, underscoring the urgent need for new therapeutic targets. This study applies an extensive subtractive proteomics approach to identify viable drug targets within the core proteome of P. aeruginosa PAO1, analyzing a total of 5563 proteins. Through a rigorous, multi-stage process, we excluded human homologs, identified essential proteins, mapped functional pathways, determined subcellular localization, and assessed virulence and resistance factors. This comprehensive analysis led to the identification of three novel, druggable targets integral to P. aeruginosa's pathogenicity and multidrug resistance: prepr

SUBMITTER: Vemula D 

PROVIDER: S-EPMC11891712 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature