Mining yeast diversity unveils novel targets for improved heterologous laccase production in Saccharomyces cerevisiae.

ABSTRACT: The budding yeast Saccharomyces cerevisiae is a widely utilized host cell for recombinant protein production due to its well studied and annotated genome, its ability to secrete large and post-translationally modified proteins, fast growth and cost-effective culturing. However, recombinant protein yields from S. cerevisiae often fall behind that of other host systems. To address this, we developed a high-throughput screen of wild, industrial and laboratory S. cerevisiae isolates to identify strains with a natural propensity for greater recombinant protein production, specifically focussing on laccase multicopper oxidases from the fungi Trametes trogii and Myceliophthora thermophila. Using this method, we identified 20 non-laboratory strains with higher capacity to produce active laccase. I

SUBMITTER: Wong RWK 

PROVIDER: S-EPMC11892151 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature