Different NGS identification methods of somatic mutation sites in solid tumors impact TMB results.

ABSTRACT:

Background

Tumor mutation burden (TMB) is a predictive biomarker for assessing the response of various tumor types to immune checkpoint inhibitors (ICI). TMB is quantified based on somatic mutations identified by next-generation sequencing (NGS) using targeted panel data. This study aimed to investigate whether different NGS methods will affect the results of TMB detection in solid tumors.

Materials and methods

In this study, a hybrid capture NGS method was performed to identify Tumor-only (TO) tissue and tumor tissue and white blood cells Tumor Control (TC). The accuracy and specificity of the two employed methods were evaluated by the identification and analysis of standard reference data. Based on the quality control of FFPE samples, 24 pathological and imaging confirmed

SUBMITTER: Chen X 

PROVIDER: S-EPMC11892244 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature