STING Deficiency Promotes Th17-Like Tfh to Aggravate the Experimental Autoimmune Uveitis.

ABSTRACT:

Purpose

The purpose of this study was to explore the underlying mechanism that Th17-like T follicular helper cells (Tfh) orchestrated by STING signaling have a pathogenic role in experimental autoimmune uveitis (EAU).

Methods

The differences of transcriptome and gene ontology (GO) pathway of Tfh between EAU and control mice were analyzed by single-cell RNA sequence (scRNA-seq) and bulk RNA sequence. Additionally, draining lymph nodes (DLNs) were extracted to verify the expression of IL-17A and IFN-γ in Tfh from EAU and control mice by flow cytometry. Then, the scRNA-seq and flow cytometry were used to explore the different proportion of Tfh between STING deficiency (Sting-/-) mice and wild type (WT) mice. In vitro, naïve CD4+ T cells were isolated from Sting-/- mice and WT m

SUBMITTER: Li Z 

PROVIDER: S-EPMC11892529 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature