Fibronectin Mediates Endothelial-to-Mesenchymal Transition in Retina Angiogenesis.

ABSTRACT:

Purpose

The purpose of this study was to investigate the role of endothelial-mesenchymal transition (EndoMT) in pathological retinal angiogenesis and identify key molecular mediators in retina angiogenesis.

Methods

RNA sequencing (RNA-seq) was performed on retinal tissue from an oxygen-induced retinopathy (OIR) mouse model to analyze gene expression patterns. The Gene Set Enrichment Analysis was used to examine the correlation between epithelial-mesenchymal transition (EMT) and angiogenesis gene sets. Fibronectin (FN1) expression was evaluated in endothelial cells, and its function was assessed through siRNA-mediated knockdown in both in vitro angiogenesis assays and the OIR model.

Results

EndoMT occurred early in retinal angiogenesis development, with significant cor

SUBMITTER: Liu D 

PROVIDER: S-EPMC11892531 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature