Single-Cell Multiomics Analysis of Early Wound Response Programs in the Mouse Corneal Epithelium.

ABSTRACT:

Purpose

Wound healing is crucial for restoring homeostasis in living organisms. Although wound response mechanisms have been studied extensively, the gene regulatory programs involved remain to be elucidated. Here, we used single-cell RNA sequencing (RNA-seq) and ATAC sequencing (ATAC-seq) analysis to profile the regulatory landscape of mouse corneal epithelium in early wound response.

Methods

We used our previously published single-cell data sets of homeostatic adult mouse corneal epithelium as the unwounded group. The wounded group data sets were obtained by sequencing the epithelium after an annular epithelial wound. Following the integration of the relevant data sets, the Seurat and ArchR packages were employed for single-cell RNA-seq and single-cell ATAC-seq data proces

SUBMITTER: Lu ZJ 

PROVIDER: S-EPMC11892537 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature