Improvements in RNA and DNA nanopore sequencing allow for rapid genetic characterization of avian influenza.

ABSTRACT: Avian influenza virus (AIV) currently causes a panzootic with extensive mortality in wild birds, poultry, and wild mammals, thus posing a major threat to global health and underscoring the need for efficient monitoring of its distribution and evolution. We here utilized a well-defined AIV strain to systematically investigate AIV genetic characterization through rapid, portable nanopore sequencing by comparing the latest DNA and RNA nanopore sequencing approaches and various computational pipelines for viral consensus sequence generation and phylogenetic analysis. We show that the latest direct RNA nanopore sequencing updates improve consensus sequence generation, but that the application of the latest DNA nanopore chemistry after reverse transcription and amplification outperforms, such na

SUBMITTER: Perlas A 

PROVIDER: S-EPMC11892550 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature