CTCF regulates global chromatin accessibility and transcription during rod photoreceptor development.

ABSTRACT: Chromatin architecture facilitates accurate transcription at a number of loci, but it remains unclear how much chromatin architecture is involved in global transcriptional regulation. Previous work has shown that rapid depletion of the architectural protein CTCF in cell culture alters global chromatin organization but results in surprisingly limited gene expression changes. This discrepancy has also been observed when other architectural proteins are depleted, and one possible explanation is that full transcriptional changes are masked by cellular heterogeneity. We tested this idea by performing multiomics analyses with sorted juvenile postmitotic mouse rods, which undergo synchronized development, and we identified CTCF-dependent regulation of global chromatin accessibility and gene expre

SUBMITTER: Chen D 

PROVIDER: S-EPMC11892594 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature