Development of a colloidal gold-based immunochromatographic strip for rapid detection of <i>Areca palm velarivirus 1</i>.

ABSTRACT:

Background

Areca palm velarivirus 1 (APV1), the causal agent of betel palm yellow leaf disease (YLD), has caused significant yield losses and decreased product quality of betel nuts, posing a serious economic threat to local farmers. There is an urgent need for a convenient and reliable method for the rapid detection and surveillance of APV1.

Methods

The Capsid protein (CP) of APV1 was expressed in Escherichia coli and purified as antigen to immunize BALB/c mice. Two specific monoclonal antibodies (MAbs), APV1CP-1 and APV1CP-10, were generated through the hybridoma technique. APV1CP-1 was conjugated with colloidal gold served as detection reagent, while APV1CP-10 was coated onto a porous nitrocellulose membrane to act as the detection line. Goat anti-mouse IgG

SUBMITTER: Lu J 

PROVIDER: S-EPMC11892987 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature