Nedd4L signaling contributes to carbon tetrachloride-induced liver fibrosis in female mice and is associated with enteric dysbacteriosis.

ABSTRACT:

Background

Liver fibrosis is characterized by hepatic stellate cell (HSC) activation and collagen overproduction, but its pathogenesis remains largely unknown. This study aimed to uncover the role of neural precursor cell expressed developmentally downregulated 4-like (Nedd4L) signaling in liver fibrosis and its relationship with gut microbiota.

Methods

Intraperitoneal injection of carbon tetrachloride (CCl₄) was used to induce liver fibrosis in 8-week-old female C57BL/6J mice with Nedd4L knockout or administration of the Nedd4L protein phosphorylation inhibitor EMD638683. HSCs isolated from mice were activated with transforming growth factor-beta 1 (TGFβ1) with or without EMD638683.

Results

An approximately 3-fold elevation in Nedd4L mRNA was

SUBMITTER: Chen C 

PROVIDER: S-EPMC11893157 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature