The <i>Pseudomonas aeruginosa sphBC</i> genes are important for growth in the presence of sphingosine by promoting sphingosine metabolism.

ABSTRACT: Sphingoid bases, including sphingosine, are important components of the antimicrobial barrier at epithelial surfaces where they can cause growth inhibition and killing of susceptible bacteria. Pseudomonas aeruginosa is a common opportunistic pathogen that is less susceptible to sphingosine than many Gram-negative bacteria. Here, we determined that the deletion of the sphBCD operon reduced growth in the presence of sphingosine. Using deletion mutants, complementation and growth assays in P. aeruginosa PAO1, we determined that the sphC and sphB genes, encoding a periplasmic oxidase and periplasmic cytochrome c, respectively, were important for growth on sphingosine, while sphD was dispensable under these conditions. Deletion of sphBCD in P.

SUBMITTER: DiGianivittorio P 

PROVIDER: S-EPMC11893366 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature