HOXB4 enforces equivalent fates of ES-cell-derived and adult hematopoietic cells.

ABSTRACT: Genetic manipulation of hematopoietic stem and progenitor cells is an important tool for experimental and clinical applied hematology. However, techniques that allow for gene targeting, subsequent in vitro selection, and expansion of genetically defined clones are available only for ES cells. Such molecularly defined and, hence, "safe" clones would be highly desirable for somatic gene therapy. Here, we demonstrate that in vitro differentiated ES cells completely recapitulate the growth and differentiation properties of adult bone marrow cells, in vitro and in vivo, when ectopically expressing HOXB4. Myeloid development was enforced and (T) lymphoid development suppressed over a wide range of expression levels, whereas only high expression levels of the transcription factor were detrimental

SUBMITTER: Pilat S 

PROVIDER: S-EPMC1189347 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature