Mutations in the ilvN gene mitigate growth inhibitory effect of cysteine in Corynebacterium glutamicum.

ABSTRACT: Cysteine, a common amino acid used in food, cosmetic, and pharmaceutical industries, has a growth inhibitory effect. This growth inhibition by cysteine poses a problem, as the production of cysteine using microbial cells results in decreased cell growth and cysteine productivity. The underlying mechanism of growth inhibition by cysteine is unclear. This study aims to understand the mechanism of growth inhibition by cysteine in Corynebacterium glutamicum. To do this, cysteine-resistant mutants of C. glutamicum were isolated based on adaptive laboratory evolution (ALE) and their characteristics were analyzed. Genome resequencing revealed that mutations in the open reading frame of the ilvN gene encoding the regulatory small subunit of acetohydroxyacid synthase (AHAS), which is involved in br

SUBMITTER: Matsuhisa K 

PROVIDER: S-EPMC11893703 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature