Establishment and validation of a simple and accurate qPCR detection method for Haemophilus parasuis.

ABSTRACT: As an infectious disease that poses a significant threat to the rapidly growing pig breeding industry, the detection of Haemophilus parasuis (HPS) is often compromised by various interfering substances present in the test sample during quantitative real-time PCR (qPCR). The rapid detection of HPS is important for the isolation of infectious pigs and their treatment. We designed and optimized a rapid qPCR test to detect the INFB gene of HPS in clinical and environmental samples on pig farms. The method was evaluated for its specificity, sensitivity, repeatability, anti-interference capability, and its ability to detect HPS in clinical samples. The results indicated that the method was specific for the detection of HPS when evaluated against pathogens and intestinal probiotics found in pig f

SUBMITTER: Jin H 

PROVIDER: S-EPMC11893755 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature