Haploinsufficient phenotypes promote selection of PTEN and ARID1A-deficient clones in human colon.

ABSTRACT: Cancer driver mutations are defined by their high prevalence in cancers and presumed rarity in normal tissues. However, recent studies show that positive selection in normal epithelia can increase the prevalence of some cancer drivers. To determine their true cancer-driving potential, it is essential to evaluate how frequent these mutations are in normal tissues and what are their phenotypes. Here, we explore the bioavailability of somatic variants by quantifying age-related mutational burdens in normal human colonic epithelium using immunodetection in FFPE samples (N = 181 patients). Positive selection of variants of tumour suppressor genes PTEN and ARID1A associates with monoallelic gene loss as confirmed by CRISPR/Cas9 mutagenesis and changes in their downstream effectors. Comparison of

SUBMITTER: Skoufou-Papoutsaki N 

PROVIDER: S-EPMC11893880 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature