Analyses of the essential C82 subunit uncovered some differences in RNA polymerase III transcription between <i>Trypanosoma brucei</i> and <i>Leishmania major</i>.

ABSTRACT: The 17-subunit RNA polymerase III (RNAP III) synthesizes essential untranslated RNAs such as tRNAs and 5S rRNA. In yeast and vertebrates, subunit C82 forms a stable subcomplex with C34 and C31 that is necessary for promoter-specific transcription initiation. Little is known about RNAP III transcription in trypanosomatid parasites. To narrow this knowledge gap, we characterized the C82 subunit in Trypanosoma brucei and Leishmania major. Bioinformatic analyses showed that the 4 distinctive extended winged-helix (eWH) domains and the coiled-coil motif are present in C82 in these microorganisms. Nevertheless, C82 in trypanosomatids presents certain unique traits, including an exclusive loop within the eWH1 domain. We found that C82 localizes to the nucleus and binds to RNAP III-d

SUBMITTER: Cano-Santiago A 

PROVIDER: S-EPMC11894013 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature