Characterization of the procoagulant phenotype of amniotic fluid across gestation in rhesus macaques and humans.

ABSTRACT:

Background

Amniotic fluid (AF) plays a key role in fetal development, yet the evolving composition of AF and its effects on hemostasis and thrombosis are poorly understood.

Objectives

To characterize the procoagulant properties of AF as a function of gestation in humans and nonhuman primates.

Methods

We analyzed the proteomes, lipidomes, and procoagulant properties of AF obtained by amniocentesis from rhesus macaque and human pregnancies at gestational age-matched time points.

Results

When added to human plasma, both rhesus and human AF accelerated clotting time and fibrin generation. We identified proteomic modules associated with clotting time and enriched for coagulation-related pathways. Proteins known to be involved in hemostasis were highly correlated wit

SUBMITTER: Yang CJ 

PROVIDER: S-EPMC11894163 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature