Geometrical constraint change determines organized collective migration of follower cells.

ABSTRACT: Spatial confinement plays a critical role in shaping collective cell migration, particularly in regulating interactions between leader and follower cells and among follower cells themselves. However, how changes in confinement geometry influence migration dynamics and cell-to-cell interactions remains poorly understood. This study leverages a novel microchannel design to systematically dissect the interplay between spatial confinement and collective cell behavior in endothelial-like cells (MILE SVEN 1). In a single-cell-wide T-shaped branching structure, rear cells selected alternate pathways, avoiding direct alignment with preceding cells. This highlights how spatial geometry mediates follower-follower interactions by encouraging dynamic rearrangements within the cell train. Ladder-like b

SUBMITTER: Sentoku M 

PROVIDER: S-EPMC11894221 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature