Integrated multi-omics characterization of neuroblastoma with bone or bone marrow metastasis.

ABSTRACT: The pathogenesis of neuroblastoma with bone or bone marrow metastasis (NB-BBM) and its complex immune microenvironment remain poorly elucidated, hampering the advancement of effective risk prediction for BBM and limiting therapeutic strategies. Feature recognition of 142 paraffin-embedded hematoxylin-eosin-stained tumor section images was conducted using a Swin-Transformer for pathological histology to predict NB-BBM occurrence. Single-cell transcriptomics identified a tumor cell subpopulation (NB3) and two tumor-associated macrophage (TAM) subpopulations (SPP1⁺ TAMs and IGHM⁺ TAMs) closely associated with BBM and highlighted transketolase (TKT) as a key molecular marker for metastatic progression in NB. This extensive multi-omics investigation into NB-BBM enhances ou

SUBMITTER: Huang K 

PROVIDER: S-EPMC11894310 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature