Neutralizing IgG₄ antibodies are a biomarker of sustained efficacy after peanut oral immunotherapy.

ABSTRACT:

Background

Clinical efficacy of oral immunotherapy (OIT) has been associated with the induction of blocking antibodies, particularly those capable of disrupting IgE-allergen interactions. Previously, we identified mAbs to Ara h 2 and structurally characterized their epitopes.

Objective

We investigated longitudinal changes during OIT in antibody binding to conformational epitopes and correlated the results with isotype and clinical efficacy.

Methods

We developed an indirect inhibitory ELISA using mAbs to block conformational epitopes on immobilized Ara h 2 from binding to serum immunoglobulins from peanut-allergic patients undergoing OIT. We tested the functional blocking ability of mAbs using passive cutaneous anaphylaxis in mice with humanized FcεRI receptors.

Res

SUBMITTER: Keswani T 

PROVIDER: S-EPMC11894811 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature