Rapidly quantification of intact infectious H1N1 virus using ICA-qPCR and PMA-qPCR.

ABSTRACT: The increase in emerging and reemerging infectious diseases has underscored the need for the prompt monitoring of intact infectious viruses and the quick assessment of their infectivity. However, molecular techniques cannot distinguish between intact infectious and noninfectious viruses. Here, two distinct methodologies have been developed for the expeditious and dependable quantification of intact infectious H1N1 virus, and several experiments have been conducted to substantiate their efficacy. One is an integrated cell absorption quantitative polymerase chain reaction (qPCR) method (ICA-qPCR), and the other is a combined propidium monoazide qPCR method (PMA-qPCR). The quantification limit is 100 cell culture infective dose 50 % (CCID₅₀)/mL in ICA-qPCR following a 1.5-hour cell

SUBMITTER: Liang C 

PROVIDER: S-EPMC11895024 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature