Unveiling radiobiological traits and therapeutic responses of BRAF^V600E-mutant colorectal cancer via patient-derived organoids.

ABSTRACT:

Background

Radiotherapy (RT) is an essential treatment for colorectal cancer (CRC), yet the factors influencing radiosensitivity remain unclear. In the quest to enhance the therapeutic efficacy in CRC, the interplay between genetic mutations and RT sensitivity has emerged as a pivotal yet enigmatic area.

Methods

We harness the fidelity of patient-derived organoids (PDOs) to dissect the molecular landscape of radiosensitivity, with a particular emphasis on BRAF^V600E mutations. To further investigate, a cohort of 9 BRAF^V600E-mutant and 10 BRAF wild-type PDOs is constructed to systematically assess the radiobiological traits of BRAF^V600E-mutant CRC, including morphology, cell viability, and DNA damage, while also evaluating their responses to ch

SUBMITTER: Mu P 

PROVIDER: S-EPMC11895145 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature