The diagnostic accuracy of preimplantation genetic testing (PGT) in assessing the genetic status of embryos: a systematic review and meta-analysis.

ABSTRACT:

Background

Preimplantation genetic testing (PGT) is widely used in assisted reproduction to assess the genetic status of embryos. However, increasing evidence suggests that the trophectoderm (TE) may not fully reflect the genetic status of the inner cell mass (ICM), raising controversy about the accuracy of TE biopsy. Research in recent years has focused on cell-free DNA (cfDNA) found in blastocoel fluid (BF) and spent culture medium (SCM), as these may contain genetic information from both the TE and ICM. Therefore, further research and validation are essential to determine the reliability and clinical applicability of these diagnostic methods in PGT.

Methods

Relevant studies published between January 2000 and August 2024 were identified through PubMed and Web of Science (W

SUBMITTER: Chen K 

PROVIDER: S-EPMC11895315 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature